Which of the following statements best justifies the inclusion of test tubes 3 and 7

indicator. A solution containing iodine and starch will have adark blue color, whereas a solution containing iodine but nostarch will have a light brown color. The color change of aniodine solution from dark blue to light brown can be used tomeasure the rate at which starch is converted to simplesugars. A student designs an experiment to investigate theeffect of environmental pllon amylase function. The design ofthe experimentis presented in Table 1 Table 1. An experimentfor investigating the effect of pH on amylase function lodineAdded Test Tube Starch Added Pretreated by BoilingAmylase Added Environmental pH! - = 2 > 5 VIII Vas controlin the experiment?Which of the following statements best justifies the inclusionof test tube V as a control in the experiment? It will provide ameasurement of amylase activity at an acidic pH. B it willprovide a measurement of amylase activity at a basic pH. c)It will show the color change that occurs in the absence ofenzyme activity. (D) it will show the color change that occursin the absence of the amylase protein.

AP BiologyTest BookletCopy of Enzyme Semester ExamNameCopyright © 2021. The College Board. These materials are part of a College Board program. Use or distribution of these materials online or in print beyond yourschool’s participation in the program is prohibited.Page 1 of 26For the following questions: Graphs I-IV depict the effect of pH on the activity of four different hydrolyticenzymes.1.Enzymes with their highest activity at an alkaline (basic) pH are represented by which of the followinggraphs?AI onlyBII onlyCIII onlyDI and III onlyEI and IV only

AP BiologyTest BookletCopy of Enzyme Semester ExamCopyright © 2021. The College Board. These materials are part of a College Board program. Use or distribution of these materials online or in print beyond yourschool’s participation in the program is prohibited.Page 2 of 262.Graphs representing enzymes sensitive to changes in pH include which of the following?AI onlyBIV onlyCII and III onlyDIII and IV onlyEI, II, and III only3.The most likely explanation for the results shown in Graph I is thatApH affects the shape of the active site of the enzymeBpH affects the temperature of the reactionCthe enzyme has a quaternary structureDthe enzyme has disulfide bondsEpH affects the primary structure of the enzyme

AP BiologyTest BookletCopy of Enzyme Semester ExamCopyright © 2021. The College Board. These materials are part of a College Board program. Use or distribution of these materials online or in print beyond yourschool’s participation in the program is prohibited.Page 3 of 264.The diagram below shows energy changes in a specific chemical reaction with and without the addition ofan enzyme to the reaction.Which of the following questions can best be answered by the diagram?ADoes the addition of an enzyme reduce the activation energy required for a reaction?BDoes the addition of an enzyme result in the formation of covalent bonds?CDoes the addition of an enzyme produce a greater amount of products?DDoes the addition of an enzyme change the pathway for the reaction?5.A researcher proposes a model of an enzyme-catalyzed reaction in which a reactant is converted to aproduct. The model is based on the idea that the reactant passes through a transition state within the enzyme-substrate complex before the reactant is converted to the product.Which of the following statements best helps explain how the enzyme speeds up the reaction?

AP BiologyTest BookletCopy of Enzyme Semester ExamCopyright © 2021. The College Board. These materials are part of a College Board program. Use or distribution of these materials online or in print beyond yourschool’s participation in the program is prohibited.Page 4 of 26AThe enzyme’s active site binds to and stabilizes the reactant, which decreases the free-energy change of thereaction.

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Which of the following statements best justifies the inclusion of test tube?

Which of the following statements best helps justify the inclusion of the test tube 5 as a control in the experiment?

Which of the following best justifies the use of tube as a control treatment?

Which of the following statements best helps justify the inclusion of group 2 as one of the controls?