Which of the following best explains the banding patterns exhibited in Figure 4

5.The restriction endonuclease Mst II recognizes the sequence 5' CCT(N)AG (where N = any nucleotide) and cutsDNA at that site, producing separate fragments. Which of the following best explains the banding patternsexhibited in Figure 4?a.The HbA DNA contains a recognition site for the Mst II restriction enzyme.b.The HbA/HbS DNA contains three recognition sites for the Mst II restriction endonuclease.c.Individual I has only one copy of the hemoglobin gene; therefore there is only one band on the gel.d.The HbS/HbA DNA contains three different alleles for sicklecell disease.

AP Biology Exam Review6.The figure to the right depicts the DNAprotein complexthat is assembled at the transcriptional start site of gene Xwhen the expression of gene X is activated in liver cells.Previous studies have shown that gene X is neverexpressed in nerve cells. Based on the diagram, which ofthe following most likely contributes to the specificexpression pattern of gene X?

7.Lactose digestion in E. coli begins with its hydrolysis by the enzyme bgalactosidase. The gene encoding bgalactosidase, lacZ, is part of a coordinately regulated operon containing other genes required for lactoseutilization.Which of the following figures correctly depicts the interactions at the lac operon when lactose is NOT beingutilized? (The legend below defines the shapes of the molecules illustrated in the options.)

AP Biology Exam Review8.Mutations in the MYO6 and POU4F3 genes have been associated with a form of hereditary hearing loss inhumans. Researchers studying the genes have proposed that POU4F3 encodes a transcription factor thatinfluences the regulation of MYO6.Which of the following questions will best help guide the researchers toward a direct test of their proposal?a.Have mutations in other genes also been associated with hearing loss?b.In what types of cells are the mutant forms of the POU4F3 gene expressed?c.Are mutations in the MYO6 and POU4F3 genes also found in mice?d.Do mutations in the POU4F3 gene affect MYO6 mRNA levels in cells?

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