All recommended PPE should be worn when collecting a specimen from a person with suspected or confirmed monkeypox. Show
Only sterile, synthetic swabs (including but not limited to polyester, nylon, or Dacron) with plastic, wood, or thin aluminum (wire) shafts should be used to collect suspected or confirmed monkeypox specimens for diagnostic testing. Do not use cotton swabs. Skin lesion material, including swabs of lesion surface, exudate, or lesion crusts are the recommended specimen types for laboratory testing of monkeypox virus specimens. Procedures and materials used for collecting specimens may vary depending on the phase of the rash (e.g., swabs from lesion surface or crust from healing lesion). Collect two swabs from each lesion, preferably from different locations on the body or from lesions that differ in appearance (e.g., a pair of swabs for each lesion with a total of 2-3 lesions). Vigorously swab each lesion, avoiding contamination of gloved hands, to ensure adequate viral DNA is collected. Unroofing or aspiration of lesions (or otherwise using sharp instruments for monkeypox testing) before swabbing is not necessary, nor recommended due to the risk for sharps injury. Place swabs from lesions, crusts, and exudate in separate tubes. See Tips for Adequate Collection of a Lesion Specimen from a Suspect Monkeypox Virus Case for additional support on specimen collection. The type of acceptable specimen (dry swab or wet swab in transport media) for diagnostic testing may vary depending on the laboratory. Contact the appropriate laboratory facility to determine the specimen types accepted. At CDC, only dry swabs or swabs in viral transport media (VTM) from lesions, or lesion crusts are currently accepted for testing. Swabs in media designated for bacterial preservation may cause PCR inhibition and are not recommended. Insert each swab into a sterile container such as a sterile tube or urine container. Glass containers are not recommended. Carefully bend to break the swab’s shaft to fit inside the sterile container (if applicable, or place the entire swab into the container) . After completely securing the lid, wipe the container with an EPA-approved disinfectant for emerging viral pathogens. Placing parafilm around the lid of the container is recommended for additional leak-proof protection, but not required. Remove gloves, wash your hands (hand hygiene), and don a new pair of gloves. Purpose: Procedure: The table below outlines the basic criteria for rejection of requests for microbiology tests. Note: All specimens not processed will be refrigerated by Laboratory Alliance for 3 days before they are discarded as a safeguard against erroneously discarding any irretrievable specimen. The charge nurse and/or physician should always be notified upon receipt of these specimens and this should be documented in the Laboratory Information Systems.
Which of the following is an acceptable sample for laboratory testing?Most often, all that is required is a blood sample. However, samples of urine, saliva, sputum, feces, semen, and other bodily fluids and tissues also can be tested.
Why would a drug urine specimen be rejected from a lab quizlet?A urine specimen may be rejected by the lab for all of the following reasons: Requisition states the specimen is catheterized, specimen contains toilet paper, and the label and requisition do not match. A cloudy specimen received in the lab may have been preserved using: Boric acid.
For which test does not require a blood sample to be collected in a prewarmed tube and kept at body temperature until delivered to the laboratory?Two hours after the patient eats. For which test does a blood sample need to be collected in a prewarmed tube and kept at body temperature until delivered to the laboratory? Cold agglutinins.
Which of the following can cause hemolysis of a blood specimen?Hemolysis can be caused by rough handling of a blood specimen, leaving the tourniquet on too long (causing blood stasis) or squeezing the tip of the finger too hard during capillary collection, dilution, exposure to contaminants, extremes in temperature, or pathologic conditions.
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