What specimen would not meet requirements and is likely to be rejected from the laboratory?

All recommended PPE should be worn when collecting a specimen from a person with suspected or confirmed monkeypox.

Only sterile, synthetic swabs (including but not limited to polyester, nylon, or Dacron) with plastic, wood, or thin aluminum (wire) shafts should be used to collect suspected or confirmed monkeypox specimens for diagnostic testing. Do not use cotton swabs.

Skin lesion material, including swabs of lesion surface, exudate, or lesion crusts are the recommended specimen types for laboratory testing of monkeypox virus specimens. Procedures and materials used for collecting specimens may vary depending on the phase of the rash (e.g., swabs from lesion surface or crust from healing lesion). Collect two swabs from each lesion, preferably from different locations on the body or from lesions that differ in appearance (e.g., a pair of swabs for each lesion with a total of 2-3 lesions). Vigorously swab each lesion, avoiding contamination of gloved hands, to ensure adequate viral DNA is collected.   Unroofing or aspiration of lesions (or otherwise using sharp instruments for monkeypox testing) before swabbing is not necessary, nor recommended due to the risk for sharps injury. Place swabs from lesions, crusts, and exudate in separate tubes. See Tips for Adequate Collection of a Lesion Specimen from a Suspect Monkeypox Virus Case for additional support on specimen collection.

The type of acceptable specimen (dry swab or wet swab in transport media) for diagnostic testing may vary depending on the laboratory. Contact the appropriate laboratory facility to determine the specimen types accepted. At CDC, only dry swabs or swabs in viral transport media (VTM) from lesions, or lesion crusts are currently accepted for testing. Swabs in media designated for bacterial preservation may cause PCR inhibition and are not recommended.

Insert each swab into a sterile container such as a sterile tube or urine container. Glass containers are not recommended. Carefully bend to break the swab’s shaft to fit inside the sterile container (if applicable, or place the entire swab into the container) . After completely securing the lid, wipe the container with an EPA-approved disinfectant for emerging viral pathogens. Placing parafilm around the lid of the container is recommended for additional leak-proof protection, but not required. Remove gloves, wash your hands (hand hygiene), and don a new pair of gloves.

Purpose:
This policy defines conditions that would render a specimen unacceptable for processing in Microbiology.

Procedure:
All rejected specimens are documented in the lab computer (LIS) with date, time, and the charge nurse and/or physician notified. The procedure for cancelling an unsatisfactory specimen in the LIS should be followed. Specimens which are processed as they are "one of a kind" or for some other reason should be documented in the computer under specimen comments.

The table below outlines the basic criteria for rejection of requests for microbiology tests. Note: All specimens not processed will be refrigerated by Laboratory Alliance for 3 days before they are discarded as a safeguard against erroneously discarding any irretrievable specimen. The charge nurse and/or physician should always be notified upon receipt of these specimens and this should be documented in the Laboratory Information Systems.

CATEGORY CRITERION FOR REJECTION ACTION

Identification

Container not identified

Write "container not identified” and call ordering unit to send someone to verify the ID if the request form is attached to the specimen. Otherwise, do not process unless "one of a kind". See policy below.

 

Container and request form have different ID's

Document in LIS. Call ordering floor or physician office to send correct request forms or resolve the problem.

Request form

Insufficient information marked on form

Call ordering floor or physician office for additional necessary information

     

Specimen

Specimen grossly contaminated.

Call ordering floor or physician to send repeat specimen. If they are unable to re-collect, ask them to come down to clean it in the laboratory. Final resort: clean it with germicide, wear gloves, work in bio-hazard chamber.

 

Specimen submitted in improper container.

Notify ordering floor or physician office of problem. If unable to re-collect, perform test if possible and make note in LIS that improper container was used. If unable to perform test, document reason in LIS and discard specimen.

 

Excessive delay between specimen collection and arrival in laboratory.

If specimen was not stored properly, notify ordering unit and request repeat specimen. Do not process unless patient care is likely to be compromised by delay. Note in LIS that delay may result in less accurate results.

 

Inadequate specimen for number of tests requested

Call physician for priority of requests and to request additional material. Note in LIS that specimen had inadequate volume as reason for lack of performance of certain tests.

 

Some factor renders specimen inadequate for request.
Examples: sputum on a swab or in tissue paper, stool for ova and parasites with gross barium, visible; specimen placed into formalin; container broke or leaked during transport

Call ordering floor or physician office to inform them of problem. Note the problem in LIS and do not process specimen.

 

Pooled 24 hour urine or sputum received for culture

Call ordering floor or physician office to inform them that 24 - hour specimens are unacceptable for microbiology. Do not process and send report out with note indicating reason for not performing test.

 

Dry swab received for culture

Notify ordering floor or physician office that dry swabs are not suitable for culture. Do not process. Sent report out with note indicating reason for not performing test.

 

Improper specimen for test. Examples: blood in serum tube with clot for malaria smear; slide with material smeared on for culture; pleural fluid for serology test.

Call ordering floor or physician office to verify test request. Do not process, and send documented reason for not performing test in LIS

 

Unsatisfactory expectorated sputum.

See sputum screening procedure.

 

Formed stool for C. difficile toxins

See stool rejection policy of C. difficile

 

Foley catheter tip

Call ordering floor or physician office to inform them that clinically relevant results cannot be obtained from this specimen. Request a urine for culture. Send out report noting the reason for not processing the specimen.

 

Duplicate specimen (other than duplicate blood)

Send notice that only one of the received specimens will be processed unless the laboratory is notified and reasons are given for processing duplicate(s).

 

Anaerobic culture requested on improper specimen. Examples: expectorated sputum, voided urine (not suprapubic tap), vaginal discharge, prostatic secretions, gastrointestinal tract (except in blind loop syndrome), oral material, respiratory secretions not collected by needle aspiration or by special plugged double-Lumen catheter device, lochia, environmental material, any specimen exposed to air for extended time.

Notify ordering floor or physician office that the specimen is unacceptable for such a request. Process aerobically only.
 

IUD

Sample submitted for Amplified Molecular tests such as C. diff, GC, chlamydia.

Inappropriate for culture as contaminated with normal vaginal flora

A. Previous specimen negative within 7 days.
B. Previous specimen positive within 28 days.

Which of the following is an acceptable sample for laboratory testing?

Most often, all that is required is a blood sample. However, samples of urine, saliva, sputum, feces, semen, and other bodily fluids and tissues also can be tested.

Why would a drug urine specimen be rejected from a lab quizlet?

A urine specimen may be rejected by the lab for all of the following reasons: Requisition states the specimen is catheterized, specimen contains toilet paper, and the label and requisition do not match. A cloudy specimen received in the lab may have been preserved using: Boric acid.

For which test does not require a blood sample to be collected in a prewarmed tube and kept at body temperature until delivered to the laboratory?

Two hours after the patient eats. For which test does a blood sample need to be collected in a prewarmed tube and kept at body temperature until delivered to the laboratory? Cold agglutinins.

Which of the following can cause hemolysis of a blood specimen?

Hemolysis can be caused by rough handling of a blood specimen, leaving the tourniquet on too long (causing blood stasis) or squeezing the tip of the finger too hard during capillary collection, dilution, exposure to contaminants, extremes in temperature, or pathologic conditions.