Magnification:
Return To Top of Page Show Microscope Notes Note: A compound microscope has 2 sets of lens. This kind of microscope is also called a light microscope as it requires a source of light to pass through the specimen.
Microscope Lighting 1. Under low power, the field of view is? a. dark b. bright 2. If it’s too bright under low power, what should you do? 3. As you switch from low to high power, the field of view becomes? a. darker with a decrease in diameter b. brighter with an increase in diameter 4. If it’s too dark under high power, what should you do? Answers: 1. Brighter 2. Partially close the diaphragm 3. Darker with a decrease in diameter 4. The diaphragm needs to be opened to allow in more light Examining the Letter “e” on a slide Note: When a wet mount of the letter “e” is made the following steps must be followed:
Answers: 1. Image is reversed & inverted. 2. Moves to the opposite direction. 3. Coarse adjustment knob because the slide will crack. Making a wet mount of an onion cell Note: When making a wet mount of an onion cell the following steps must be followed: 1. Place the specimen on a slide 2. Add a drop of water or stain. 3. Place a cover slip at an angle so that it touches the drop. 4. Lower the raised end of the cover slip slowly. The diagram below shows that as the cover slip is lowered, the drop of liquid moves to the right. Staining an onion cell Note: Staining an onion allows you to better observe structural parts of a plant cell. The staining reagents used are either iodine (Lugol’s solution) or methylene blue. 1. Prepare a wet mount (follow the above steps). 2. Place one drop of iodine alongside the coverslip.
Magnification Power of the Eyepiece (ocular) X Power of the objective lens = Total Magnification of the Light Microscope Measuring a specimen viewed under a microscope
Rule: 1,000 micrometers = 1 mm (millimeter) 2. The second image on the right is of the same organism as the first image on the left. What happened to form the image on the right?
Answer: The student is viewing the same ameba on high power. The field of view gets smaller, which makes the ameba appear larger in this field.What is the total magnification of your microscope using the high power objective?High Power Objective Lens (40x)
The total magnification of a high-power objective lens combined with a 10x eyepiece is equal to 400x magnification, giving you a very detailed picture of the specimen in your slide.
What is the magnification of high power microscope?High power microscopes – also called compound microscopes because of their two-stage magnification – are the 'typical' microscope that is seen in laboratories and on television. High power microscopes typically magnify in the range ×40 to ×1000; much greater than this will only produce fuzzy images with no more detail.
What is the total magnification at 4x 10x and 40x?
Which objective lens has a magnification of 10x?Low Power Lens (10x)
The low power objective lens usually has 10x or 20x magnification.
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