Osmosis is a special case of diffusion in which water molecules pass through a selectively permeable membrane, but larger molecules do not. Osmosis proceeds from a region of high water concentration, across a semi-permeable membrane, to a region of lower water concentration until equilibrium is reached. A solute is a solid substance, such as salt or sugar that is dissolved in a solvent. Water is usually the solvent in living systems. A typical animal cell contains a salt concentration of 0.9%. A solution of equal solute concentration is referred to as isotonic. A cell placed in an isotonic environment will experience movement of water inside and outside the cell, but there will be no change A
hypertonic solution contains a high solute concentration with respect to cells. For example, a solution containing 10% salt is hypertonic. When a cell is placed in a hypertonic environment, there is a net movement of water to the outside of the cell (from the higher water environment inside the cell). The cell shrinks in response. A solution of low solute concentration is referred to as hypotonic. A solution containing 0.5% salt is hypotonic with respect to the cell. When a cell is placed in a hypotonic environment, there is a net movement of water into the cell. The cell swells in response. Hypertonic, hypotonic, and isotonic are relative terms and are used only when comparing two different solutions. Osmosis in eggplant and potato cellsMaterials
Procedure
Add a potato piece to each tube and incubate at room temperature for ~15 minutes Pour off the solution and feel each potato piece. Rinse test-tubes thoroughly with water to remove traces of salt and potato starch ObservationsIf you're seeing this message, it means we're having trouble loading external resources on our website. If you're behind a web filter, please make sure that the domains *.kastatic.org and *.kasandbox.org are unblocked.
What happens when red blood cells are placed in 10% NaCl solution?But a 10% salt solution is hypertonic to the cell cytoplasm, so the water will move out of the cell due to osmosis. This will result in shrinkage of the red blood cells resulting in collapse of the cell.
What happened to the cells to cause them to shrink?If a cell is placed in a hypertonic solution, water will leave the cell, and the cell will shrink. In an isotonic environment, there is no net water movement, so there is no change in the size of the cell. When a cell is placed in a hypotonic environment, water will enter the cell, and the cell will swell.
What happens to blood cells in NaCl solution?When subjected to hypertonic media (e.g. 1.8% NaCl), the cells lose their normal biconcave shape, undergoing collapse (leading to crenation) due to the rapid osmotic efflux of water.
Why would red blood cells placed in a 10% salt solution appear wrinkled?When red blood cells are in a hypertonic (higher concentration) solution, water flows out of the cell faster than it comes in. This results in crenation (shriveling) of the blood cell.
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